Effects of Cirsium setidens extract on gene expression related to apoptosis induction and metastasis inhibition in human breast cancer

Cell apoptosis induction and metastasis suppression in human breast cancer cell, MDA-MB-231, were studied to assess the potential of Cirsium setidens extract (CSE) on treatment. viability analysis using MDA-MB-231 confirmed CSE significantly inhibited growth proliferation cell without suppressing normal range 0.25 0.5 mg/mL CSE. To analyze anticancer effect CSE, expressions major genes related apoptosis, including p53, AMPK-α, Bcl-2, Bax analyzed by reverse transcription-polymerase chain reaction. The increased 45, 43, 68%, respectively, while expression Bcl-2 decreased 12%, showing that treatment induced MDA-MB-231. compound was identified as rhoifolin, a type flavone, through LC-MS/MS. Our findings known possess variety significant biological activities, could be obtained from C. used an effective natural suppress cancer.